abstract：:The authors describe a method of estimation of clometacine in biological fluids based on extraction in the pure state of a pair of ions, purification by chromatography (in liquid phase, adsorption, then exchange, methylation by diazo-methane and gas chromatography associated with detection by electron capture. ...

journal_title：Annales de biologie clinique

authors： Bacle B,Bourdon R

更新日期：1978-01-01 00:00:00

abstract：:The indirect fluorescent antibody (IFA) technique for specific IgM anti-Candida albicans was applied to 158 sera and compared with sucrose density gradient centrifugation. For detection of specific IgM results of both methods were in agreement for 157 sera (99.36%). Specific IgM titers determined with IFA on unfractio...

journal_title：Annales de biologie clinique

authors： Guinet R,Absi N

更新日期：1978-01-01 00:00:00

abstract：:Free and total tryptophan is estimated in the plasma by a spectrofluorimetric method using an amplification reaction and fluorescence by transformation of tryptophan into norharman using formalin and perhydrol (fluorescence is amplified about 15 times). Free tryptophan in the plasma is separated by ultrafiltration und...

journal_title：Annales de biologie clinique

authors： Escande C,Bousquet B,Dreux C

更新日期：1977-01-01 00:00:00

abstract：:Bacillus alvei hemolytic toxin has been characterized in five main steps from a 18 hours culture supernatant fluid. 50% ammonium sulfate precipitations are followed by Sephadex gel filtrations. After that we proceed to an isoelectric focusing in a narrow pH gradient (from 4 to 6). A final step consists in a gel filtra...

journal_title：Annales de biologie clinique

authors： Kiredjian M

更新日期：1977-01-01 00:00:00

abstract：:An automated kinetic method to determine creatinine in plasma with Jaffé reaction (alkaline picrate reagent) using a Gemsaec analyser is studied in order to argue the precision and the accuracy. The analysis of the reaction rate of a primar standard solution and of different plasmas suggests that a rapid measure is re...

journal_title：Annales de biologie clinique

authors： Klumpp T,Férard G

更新日期：1977-01-01 00:00:00

abstract：:The analytical modifications necessary for adaptation of the SFBC technic for measurement of the activity of TGO method and those obtained by the technic adapted on ABA 100 suggest that the modifications proposed have no influence on the result of the enzyme estimation. The activation of TGO by addition of its coenzym...

journal_title：Annales de biologie clinique

authors： Gobbels G,Plomteux G

更新日期：1977-01-01 00:00:00

abstract：:The present importance of streptococcal pathology implicates the identification of the streptococcal groups. The authors have carried out a comparative study of the various methods of extraction of the group specific antigen. They demonstrated the advantages and disadvantages of each method and consider the pronase ex...

journal_title：Annales de biologie clinique

authors： Bergogne-Berezin E,Slim A

更新日期：1976-01-01 00:00:00

abstract：:The authors describe a method of simultaneous estimation of phenobarbitone, mephenytoin and diphenylhydantoin in the blood. After extraction with ether, the methylation of these substances by trimethylanilinium hydroxide permits their rapid separation by gas chromatography. Heptabarbitone is used as internal standard ...

journal_title：Annales de biologie clinique

authors： Maitrot B,Hue G,Canonne MA,Rocher A,Landriau N

更新日期：1976-01-01 00:00:00

abstract：:The most generalized methods in enzymology are based on the quantitative assay of compounds, substrate or coenzyme, by spectrophotometry without any separation. Such a method is ruled out if the colorimetric reaction is not specific of the compound. In liver enzymology, aside the classical metabolic pathways, such ass...

journal_title：Annales de biologie clinique

authors： Padieu P,Maume BF

更新日期：1976-01-01 00:00:00

abstract：:We present the distribution of cytochrome P450, the terminal oxidase of the microsomal drug-metabolizing system among the different species, organs, and its subcellular localization. Physicochemical properties, and more specially spectral properties, of this hemoprotein are reviewed. We also described the two microsom...

journal_title：Annales de biologie clinique

authors： Boudene C,Boisset M

更新日期：1975-01-01 00:00:00

abstract：:A fluorimetric, authomatical method for determination of free and total cholesterol in serum is described. Cholesterol esters are splitted into free cholesterol and fatty acids by cholesterol esterase. In the presence of oxygen free cholesterol will be transformed by cholesterol oxydase in delta 4 cholestenone with fo...

journal_title：Annales de biologie clinique

authors： Fruchart JC,Tsumbu BB,Dewailly P,Sezille G,Jaillard J

更新日期：1975-01-01 00:00:00

abstract：:The presence of A-protein was sought in strains of S. epidermidis and S. aureus, sensitive or resistant to methicillin. The technique used was that of passive hemagglutination of red cells of sheep, either normal or formolated and sensitised by anti-erythrocyte antibodies. A-protein was not found in any of the 157 str...

journal_title：Annales de biologie clinique

authors： Flandrois JP,Fleurette J,Eyraud F

更新日期：1975-01-01 00:00:00

abstract：:The authors describe an automatic method of estimation of uric acid based on the colour obtained in the presence of Gaiac resin and peroxidase, and the hydrogen peroxide produced by the action of urate oxidase on uric acid. ...

journal_title：Annales de biologie clinique

authors： Pollet J,Galliot M

更新日期：1975-01-01 00:00:00

abstract：:AFP can be measured by radioimmunoassay in 52 hours using an incubation at 18 degrees C for 48 hours and the double antibody solid phase method to separate the free labelled AFP from the labelled AFP bound to the antibodies. In these conditions, the sensitivity and precision of the assay are very satisfactory and the ...

journal_title：Annales de biologie clinique

authors： Franchimont P,Zangerle PF,Debruche ML,Proyard J,Simon M,Gaspard U

更新日期：1975-01-01 00:00:00

abstract：:The authors propose a technique of labelling of leukocytes based on the phagocytic power of these cells with regard to sulphide particles in the colloidal state labelled with 90mmTc. These labelled leukocytes maintain their normal function and can be detected in the inflammatory foci created in experimental animals in...

journal_title：Annales de biologie clinique

authors： Auvergnat JC,Simon J,Le Net R,Guiraud R,Armengaud M

更新日期：1975-01-01 00:00:00